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Fig. 8 | BMC Biology

Fig. 8

From: Loss of the interferon-γ-inducible regulatory immunity-related GTPase (IRG), Irgm1, causes activation of effector IRG proteins on lysosomes, damaging lysosomal function and predicting the dramatic susceptibility of Irgm1-deficient mice to infection

Fig. 8

Irgm1 −/− mouse embryonic fibroblasts (MEFs) and bone marrow derived macrophages (BMDMs) do not die upon IFN-γ induction. a Wild type (WT) and Irgm1 −/− MEFs were induced with 200 U/mL IFN-γ or treated with 10 mM LeuLeuOMe for 24, 48, and 72 hours, or left untreated. Cells were stained with nuclear dyes Hoechst (all cells) and propidium iodide (PI; dead cells only). Representative microscopic images of Hoechst- and PI-stained WT cells are shown. Scale bar represents 100 μM. b Quantification of 8a showing the percentage of PI-positive MEFs. Dye stained nuclei were quantified with the Volocity software; 10 pictures (5000–10000 cells) per sample were quantified and the means of three independent experiments ± standard deviation are shown. c WT and Irgm1 −/− BMDM cells were induced with 200 U/mL IFN-γ, 500 ng/mL lipopolysaccharide, 5 mM LeuLeuOMe for 24, 48, and 72 hours, or left untreated. The experiment was performed as in 8a, b; 10 pictures (5000–10000 cells) per sample were quantified and the means of three independent experiments ± standard deviation are shown

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