Fig. 5

Endogenous LIX1 expression is regulated by the FGF pathway during smooth muscle cell determination. a LIX1 whole-mount in situ hybridization of E5 control GFP- and FGF8-expressing stomachs. b RT-qPCR analysis of relative mRNA expression in E5 stomachs. Data were normalized to GAPDH expression. Normalized expression levels were converted to fold changes. Values are presented as the mean ± standard deviation of n = 4 controls vs. n = 3 FGF8-expressing stomachs. *P < 0.05 by one-tailed Mann–Whitney test. Raw data are shown in Additional file 12. c LIX1 whole-mount in situ hybridization of E6.5 control GFP- and sFGFR2b-expressing stomachs. Scale bars, 1 mm. White arrows show the domain of the sFGFR2b-expressing stomach in which the expression of LIX1 is sustained