Fig. 6

The Z-cad dual sensor enriches for CSCs better than either sensor alone. a Analysis strategy for identifying CD24/CD44 expression of HMLER cells first gated based on distinct Z-cad expression patterns. The CD24/CD44 plot shown here indicates the entire cellular population (Bulk) without prior Z-cad gating. CD24 and CD44 negative box is shown in gray and was identified using singly stained samples. CSC-enriched population (CD24^low/neg/CD44^hi) is shown in the red box. b Percentage of cells falling into CSC-enriched population (CD24^lo/negCD44^hi) first gated on indicated Z-cad sensor fluorescence patterns. Bulk represents all cells. Data were analyzed using the paired Student’s t test (n = 6 biological replicates). c HMLER cells were sorted into EMT and Bulk^–EMT groups (shown) or CSC and NCSC (not shown) and were plated and grown under tumorsphere conditions (1000 cells per well in a 96-well plate, 5 technical replicates per biological replicate, 3 biological replicates). d After 12 days, spheres >75 μm were quantified. Data were analyzed using the paired Student’s t test