Fig. 6

MamK dynamics in different genetic backgrounds. Photobleaching of mCherry-MamK was used to follow the recovery of the fluorescence corresponding to the MamK filament. The mCherry-MamK translational fusion was expressed from a plasmid with the P _mamAB promoter in the following MSR strains: (a) ∆mamK, (b) ∆mamJK, (c) MSR-1B, and (d) E. coli. The left panels show representative cells for this assay, indicating the selected bleached areas (white dashed circles) and fluorescence recovery progression. The pre-bleaching image is a composite of the bright field and fluorescence channel to display subcellular localization. The right panels show the quantification of the MamK filament fluorescence recovery over the time from the corresponding strain. Zero time was measured immediately after laser pulse. The half-time fluorescence recovery is presented as t_½ in each plot. Scale bars: 1 μm