Fig. 4

Male mating success and male sexual competitiveness. The mating success of homozygous transgenic males and that of parental J06 males (a) was evaluated in experiments with 5 males and 15 females allowed to mate for 8 h. The percentage of females that mated was determined by examining dissected spermatecae for the presence of sperm. The sexual performance of transgenic males was evaluated in competition experiments (b) containing equal numbers of mature transgenic males, wild type males, and wild type females (10:10:10) in a cage for 20 h. The offspring of individual females was examined with a fluorescence microscope to determine paternity as fluorescent larvae are obtained from females mating to transgenic males and non-fluorescent larvae resulted from mating to J06 males. The mean male competitiveness index (MCI) ± standard deviation are shown for three independent experiments. An MCI of 0.5 indicates that the transgenic strain males are equally competitive with the control strain