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Table 1 Dissociation constants (K d) for interactions between Cbl variants and UbcH5B–Ub

From: Casitas B-lineage lymphoma linker helix mutations found in myeloproliferative neoplasms affect conformation

Immobilized GST-Cbl variant

Analyte

K d (μM)

WT

UbcH5B–Ub

84 ± 2

Y371F

UbcH5B–Ub

84 ± 2

Y371H

UbcH5B–Ub

39 ± 1

Y371C

UbcH5B–Ub

36 ± 1

Y371A

UbcH5B–Ub

30 ± 1

Y371S

UbcH5B–Ub

16 ± 0.7

Y371D

UbcH5B–Ub

10 ± 0.5

Y371E

UbcH5B–Ub

9 ± 0.4

pY371 Y368F

UbcH5B–Ub

2.6 ± 0.6

Y368F

UbcH5B–Ub

49 ± 2

M222E

UbcH5B–Ub

14 ± 0.6

  1. Standard errors of the mean are indicated
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BMC Biology

ISSN: 1741-7007

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