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Table 5 Crystallization conditions

From: Molecular insights into substrate recognition and catalytic mechanism of the chaperone and FKBP peptidyl-prolyl isomerase SlyD

Complex Precipitant Buffer Salt
TtSlyDFL:T1 13.2 % PEG1500 0.1 M HEPES pH 7.5 0.05 M NaCl
TtSlyDFL:S2 20 % PEG3350 0.1 M Bis Tris pH 5.5 0.2 M (NH4)2SO4
TtSlyDFL:S2-W23A 19 % PEG3350 8 % Tacsimate pH 5.0
TtSlyDFL:S2-plus2 20 % PEG6000 0.1 M Citric acid pH 5.0
TtSlyDFL:FK506 25 % PEG 3350 0.1 M Bis Tris pH 5.5 0.2 M MgCl2
TtSlyD∆IF:S2-W23A 28 % PEG400 0.1 M HEPES pH 7.5 0.2 M CaCl2
TtSlyD∆IF:S3 25 % PEG6000 0.1 M Tris HCl pH 8.0 0.2 M CaCl2
TtSlyD∆IF:FK506 20 % PEG6000 0.1 M CH3O2Na pH 5.0 0.2 M ZnCl2
  1. No additives were used except for TtSlyD:T1, where 11 % glycerol was present in the crystallant