Skip to main content

Table 5 Crystallization conditions

From: Molecular insights into substrate recognition and catalytic mechanism of the chaperone and FKBP peptidyl-prolyl isomerase SlyD

Complex

Precipitant

Buffer

Salt

TtSlyDFL:T1

13.2 % PEG1500

0.1 M HEPES pH 7.5

0.05 M NaCl

TtSlyDFL:S2

20 % PEG3350

0.1 M Bis Tris pH 5.5

0.2 M (NH4)2SO4

TtSlyDFL:S2-W23A

19 % PEG3350

8 % Tacsimate pH 5.0

–

TtSlyDFL:S2-plus2

20 % PEG6000

0.1 M Citric acid pH 5.0

–

TtSlyDFL:FK506

25 % PEG 3350

0.1 M Bis Tris pH 5.5

0.2 M MgCl2

TtSlyD∆IF:S2-W23A

28 % PEG400

0.1 M HEPES pH 7.5

0.2 M CaCl2

TtSlyD∆IF:S3

25 % PEG6000

0.1 M Tris HCl pH 8.0

0.2 M CaCl2

TtSlyD∆IF:FK506

20 % PEG6000

0.1 M CH3O2Na pH 5.0

0.2 M ZnCl2

  1. No additives were used except for TtSlyD:T1, where 11 % glycerol was present in the crystallant