From: Oligomeric interface modulation causes misregulation of purine 5´-nucleotidase in relapsed leukemia
Relative abundance (wild type compared to R367Q) | ||||||
---|---|---|---|---|---|---|
Protein:BS2G molar ratio | Protein:BS3 molar ratio | |||||
Peptide | Modified residues | ATP | 1:20 | 1:50 | 1:20 | 1:50 |
343–361 | Lys 344; Lys 359 | – | 77-23 ± 20 | 88-12 ± 12 | 47-53 ± 24 | 65-35 ± 1 |
+ | 88-12 ± 12 | 89-11 ± 11 | 62-38 ± 33 | 52-48 ± 43 | ||
345–362 | Lys 359; Lys 361 | – | 37-63 ± 4 | 48-52 ± 13 | 20-80 ± 11 | 19-81 ± 5 |
+ | 55-45 ± 5 | 43-57 ± 23 | 53-47 ± 5 | 44-56 ± 3 | ||
345–361 | Lys 359 | – | 24-76 ± 11 | 34-66 ± 15 | 17-83 ± 12 | 14-86 ± 14 |
+ | 24-76 ± 8 | 32-68 ± 18 | 30-70 ± 8 | 17-83 ± 8 | ||
343–359 | Lys 344 | – | 80-20 ± 16 | 82-18 ± 10 | 94-6 ± 4 | 76-24 ± 13 |
+ | 84-16 ± 16 | 85-15 ± 4 | 100-0 | 93-7 ± 7 |