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Table 2 Differentially modified lysine residues revealed from mass spectrometric study of protein crosslinking in the presence and absence of ATP

From: Oligomeric interface modulation causes misregulation of purine 5´-nucleotidase in relapsed leukemia

   

Relative abundance (wild type compared to R367Q)

   

Protein:BS2G molar ratio

Protein:BS3 molar ratio

Peptide

Modified residues

ATP

1:20

1:50

1:20

1:50

343–361

Lys 344; Lys 359

77-23 ± 20

88-12 ± 12

47-53 ± 24

65-35 ± 1

+

88-12 ± 12

89-11 ± 11

62-38 ± 33

52-48 ± 43

345–362

Lys 359; Lys 361

37-63 ± 4

48-52 ± 13

20-80 ± 11

19-81 ± 5

+

55-45 ± 5

43-57 ± 23

53-47 ± 5

44-56 ± 3

345–361

Lys 359

24-76 ± 11

34-66 ± 15

17-83 ± 12

14-86 ± 14

+

24-76 ± 8

32-68 ± 18

30-70 ± 8

17-83 ± 8

343–359

Lys 344

80-20 ± 16

82-18 ± 10

94-6 ± 4

76-24 ± 13

+

84-16 ± 16

85-15 ± 4

100-0

93-7 ± 7

  1. Relative abundance refers to quantitative comparison between products obtained from wild-type cN-II and the R367Q mutant. Each value represents the mean and standard deviation from three experiments