Fig. 2From: Geminin prevents DNA damage in vagal neural crest cells to ensure normal enteric neurogenesisAblation of Gem from early pre-ENCCs results in robust DNA damage response and apoptotic cell death. Cryosections at the level of the foregut of control (a–c) and Wnt1Cre|Gem (d–f) E9.5 embryos immunostained for GFP (green), γH2AX (cyan) and processed for TUNEL (red). Insets correspond to the area in the panels indicated with arrows. g, Quantification of NC (GFP+) cells that have DNA damage (γH2AX+), undergo apoptosis (TUNEL+) or both (γH2AX+;TUNEL+). Multiple t-tests without correction, **P value < 0.01. Scale bars: (a–f) 100 μm, (insets) 10 μmBack to article page