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Fig. 5 | BMC Biology

Fig. 5

From: Angiopoietin 2 signaling plays a critical role in neural crest cell migration

Fig. 5

Gain and loss of function of Ang2 in neural crest cells disrupt neural crest patterning. Transverse sections of HH St15 embryo that has been injected with DiI (red) as well as a vector (green) containing GFP (a), Ang2-FL (b), or DiO and Ang2-shRNA (c). Neural crest can be seen in inhibitory zones at the asterisk in b. d Percent of total r4 neural crest cells found in atypical locations between control (n = 7) and Ang2-FL (n = 10), p = 0.007, and between control and Ang2-shRNA (n = 11), p = 0.06, and between Ang2-FL and Ang2-shRNA, p = 0.01. All error bars indicate SEM, measurements calculated per embryo. e Measurements on the shape of the whole neural crest stream. The width was measured above the OV at the narrowest part of the stream. Comparison between control (n = 12) and Ang2-FL (n = 10), p = 0.01; control and Ang2-shRNA (n = 8), p = 0.003; and Ang2-FL and Ang2-shRNA, p = 0.04 measurements calculated per embryo. f–h Dorsal view of whole embryo at HH St15 labeled similarly to above images. Asterisks indicate neural crest in prohibitory zones. Scale bars 50 μm

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