Fig. 3

Deletion of SUT457 accelerates senescence in telomerase-deficient cells. a Schematic representation of the SUT457 locus on Chromosome III. Arrows indicate direction of transcription. b Expression levels of SYP1, SUT457, SNR65 and RPS14A determined by qRT-PCR using total RNA extracted from isogenic wild-type and sut457Δ strains. The expression levels of each gene were normalised to the expression of ACT1. Error bars represent standard error of the mean, resulting from three independent replicates. *** P < 0.001; n/s, not significant (generated by t-test). c Genetic interactions of SUT457 associated with the gene ontology term telomere organisation. Gene names in bold represent negative genetic interactions while gene names in regular font correspond to positive genetic interactions. d Telomere length analysis of genomic DNA isolated from the indicated wild-type and mutant strains at passage 1. The extracted DNA was fragmented with XhoI and subjected to denatured southern blotting using a biotinylated probe against telomeric repeats. e Senescence assays performed using liquid cultures of the indicated isogenic wild-type and mutant strains. The strains were generated through tetrad dissection of the heterozygous diploid double mutant SUT457/sut457ΔTLC1/tlc1Δ. This plot represents one of three independent tetrads examined (see also Additional file 9: Figure S4). f Telomere length analysis performed as in (d) using genomic DNA isolated from the indicated wild-type and mutant strains during passages 1 and 8