Fig. 7

Phenotype analysis of atring1a;atring1b;wus-8 and atring1a;atring1b;clv3-2 triple mutants. a–f Phenotype of atring1a;atring1b;wus-8 triple mutant flower. a A typical wus flower showing reduced floral organs and absence of carpel. b A typical atring1a;atring1b;wus-8 triple mutant flower showing increased number of sepals and petals, but still lacking central carpel. c Absence of central carpel but production of filamentous extra organs in atring1a;atring1b;wus-8 triple mutant flower. d Filamentous organ produced from the base of sepal. e Filamentous organ with a long branch curled inside a carpelloid sepal. f Ectopic carpel-like structure developed from outer whorls of atring1a;atring1b;wus-8 triple mutant flower. Arrow indicates a branch mimicking an ovule outgrowth. g–k Gynoecium phenotype of atring1a;atring1b;clv3-2 triple mutant flower. g A typical clv3-2 gynoecium fused with four carpels. h Increased carpel number and (i) overproliferated stigmatic papilla-like structures in atring1a;atring1b;clv3-2 triple mutant. j Abundant carpel-like structures outgrown from inside of gynoecium. k Stigmatic papilla-like structures overproliferating at top of gynoecium. Bars = 1 mm, except 200 μm in c–f. l Elevated WUS expression in floral buds of atring1a;atring1b;clv3-2 triple mutant compared with segregated atring1a;atring1b sibling detected by qRT-PCR (Student’s t test, **p < 0.01). Error bars represent SD from three biological replicates