From: Structural basis for potency differences between GDF8 and GDF11
 | GDF11:FS288 (native)a | apo-GDF8 (native)a | apo-GDF11 (native)a |
---|---|---|---|
Data collection | |||
Space group | P 21 21 21 | P 21 21 21 | P 32 2 1 |
Unit cell dimension | |||
  a, b, c (Å) | 56.0, 59.1, 288.5 | 29.6, 77.7, 119.5 | 65.2, 65.2, 101.9 |
  α, β, γ (°) | 90, 90, 90 | 90, 90, 90 | 90, 90, 120 |
 Wavelength (Å) | 1.03321 | 0.97856 | 1.10537 |
 Resolution (Å) | 2.35 (2.43-2.35) | 2.25 (2.32-2.25) | 1.9 (1.94-1.9) |
  R merge | 0.076 (0.519) | 0.079 (1.102) | 0.069 (0.706) |
  R pim | 0.046 (0.311) | 0.032 (0.436) | 0.032 (0.316) |
 Mn (I/σI)b | 10.0 (2.6) | 18.2 (2.0) | 10.4 (2.0) |
 CC1/2 c | 0.74 | 0.72 | 0.996 |
 Completeness (%) | 100.0 (100.0) | 100.0 (100.0) | 100.0 (100.0) |
 Redundancy | 3.7 (3.8) | 7.1 (7.3) | 5.8 (5.9) |
Refinement | |||
 Resolution (Å) | 41.28-2.35 | 36.96-2.25 | 49.37-1.9 |
 No. reflections | 40,978 (4,031) | 13,776 (1,912) | 20,166 (2,642) |
  R work (%), R free d (%) | 20.3/24.7 | 23.4/27.7 | 21.7/25.8 |
No. of atoms (molecules) | |||
 Protein | 6047 (784) | 1443 (187) | 1395 (181) |
 Water | 170 (170) | 30 (30) | 43 (43) |
 Citrate | 91 (7) |  |  |
 Phosphate | 15 (3) |  |  |
 2-Methyl-2,4-pentanediol |  | 56 (7) |  |
 B-factors (average, Å2) | 60.0 | 65.1 | 60.3 |
RMS deviations | |||
 Bond lengths (Å) | 0.005 | 0.003 | 0.007 |
 Bond angles (°) | 0.960 | 0.951 | 0.853 |
Ramachandran plot | |||
 Favored (%) | 97.15 | 94.44 | 97.63 |
 Allowed (%) | 2.59 | 5.56 | 2.37 |
 Outliers (%) | 0.26 | 0.0 | 0.0 |
 Clashscoree | 4.18 | 6.83 | 3.33 |