Fig. 4From: Functional studies of Drosophila zinc transporters reveal the mechanism for zinc excretion in Malpighian tubulesBoth Zip71B and ZnT35C expressions are responsive to zinc. a Real-time analysis of mRNA levels of Zip71B and ZnT35C in dissected Malpighian tubules of wild-type animals cultured with normal food or 1 mM zinc-supplemented food. Zip71B was significantly induced by 1 mM zinc (** P < 0.01), as ZnT35C (** P < 0.01). Means ± SEM; n = 3, two-tailed Student’s t test. b Immunostaining reveals that Zip71B is upregulated by dietary zinc addition in Malpighian tubules (arrows). Scale bars = 50 μmBack to article page