Fig. 6

Tubule-specific dZnT1 RNAi flies display resistance to high dietary zinc. a Survival rate of dZnT1-RNAi flies on different food conditions. There is no effect on survival of dZnT1-RNAi flies on normal and zinc-chelated (100 μM TPEN) food compared to control flies. However, dZnT1-RNAi flies survived significantly better on high dietary zinc (10 and 15 mM zinc) compared to wild-type flies. b Knocking down dZnT1 in the tubule could not suppress zinc sensitivity of Zip71B-RNAi flies. Data are presented as means ± SEM; n = 6. * P < 0.05, ** P < 0.01, two-tailed Student’s t test. Genotypes of the flies used are NP1093-GAL4/+ (control fly), dZnT1-RNAi/+; NP1093-GAL4/+ (dZnT1-RNAi fly), Zip71B-RNAi/+; NP1093-GAL4/+ (Zip71B-RNAi fly) and dZnT1-RNAi/Zip71B-RNAi; NP1093-GAL4/+ (Zip71B, dZnT1-RNAi fly)