Fig. 2

OsLG3 mined by Ho-LAMap and its functional validation. a Identification of the causal SNPs of QTL qGL3-3 using Ho-LAMap. Manhattan plot for candidate region association mapping for qGL3-3 (top) and Ho index plots for qGL3-3 (middle). The bottom region corresponds to the candidate gene (OsLG3), the red lines label the region for significant signal. b OsLG3 expression levels in developing panicles from NIP and NIL(SLG) plants. YP3-YP22, young panicles at 3–22 cm in length; n = 3. c Grains from NIP, NIL(SLG), three OsLG3-overexpressing lines and three RNAi-OsLG3 lines. Scale bar, 5 mm. Grain length (d), 1000-grain weight (e), grain yield per plant (f) of NIP and NIL(SLG) plants. All phenotypic data in d–f were measured from plants grown with 20 × 20 cm spacing in paddies under normal cultivation conditions. Data represent mean ± SEM (n = 30). Student’s t tests were used to generate P values