Fig. 5
From: TriPer, an optical probe tuned to the endoplasmic reticulum tracks changes in luminal H2O2
Fluorescence lifetime reports on the redox status of HyPer and TriPer. a–c Fluorescence intensity-based (in grayscale) and lifetime images (color-coded to the scale of the histogram on the right) of RINm5F cells expressing TriPerER (a), HyPercyto (b), or HyPerER before and after exposure to H2O2 (0.2 mM, 15 min) (c). A histogram of the distribution of lifetimes in the population of cells is provided (right). d Bar diagram of the fluorescence lifetime peak values from (a–c, mean ± SD, ***p < 0.005, n ≥ 10)