Fig. 1

Electrophysiological recording of the activities of the SsTRIC channel reconstituted on liposomes. a Representative recordings of the wild-type SsTRIC measured at +60 mV. Three major open states are observed in this patch, presumably attributed to the opening of one (O1), two (O2), and three (O3) monomeric pores within an SsTRIC trimer. b The all-points amplitude histogram of the electrophysiological data of the wild-type SsTRIC channel recorded at +60 mV. The bin width is set at 0.03 pA/bin. The histograms were fitted with four Gaussian functions. The four peaks correspond to the closed state (C), one monomer open (O1), two monomers open (O2), and three monomers open (O3). c The current-voltage relationships of the SsTRIC channel at O1, O2, and O3 states. The error bars indicate the standard errors of mean values (SEM). The number of measurements (n) is labeled above or below the data points