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Fig. 4 | BMC Biology

Fig. 4

From: Matriptase zymogen supports epithelial development, homeostasis and regeneration

Fig. 4

Zymogen-locked matriptase induces epidermal prostasin processing. Protein extracts from skin (a), kidney (b), lung (c), and intestine (d) from newborn St14 zym/zym (lanes 1 and 2), St14 +/+ (lanes 3 and 4), and St14 –/– (lane 5) littermates were separated by capillary electrophoresis and probed with antibodies against matriptase (top panels), prostasin (middle panels), or β-actin (bottom panels). Lanes 6 and 7 in (a) are skin extracts from prostasin null (Prss8 –/–) and prostasin zymogen-locked (Prss8 zym/zym) mice, respectively. Zymogens of matriptase and prostasin are indicated with filled arrows, and the activated forms are indicated with open arrows. n.s. non-specific. Positions of molecular weight markers (kDa) are indicated on the left. e Representative example of quantification of activated prostasin (open arrow) and zymogen prostasin (filled arrows) in protein extracts from skin from a newborn St14 zym/zym mouse (top panel), St14 +/+ mouse (second panel from top), and a newborn St14 –/– mouse (second panel from bottom). Skin extracts from a newborn mouse expressing zymogen-locked (Prss8 zym/zym) endogenous prostasin is included as reference (bottom panel). f Ratio of activated prostasin to total prostasin in skin extracts from newborn St14 zym/zym (left bar, n = 7), St14 +/+ (middle bar, n = 7), and St14 –/– (right bar, n = 2) mice, quantified as in (e). Data are shown as mean ± SD. *P = 0.0011 was determined by one-way ANOVA, two-tailed. Additional file 1: Raw supporting data

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