Fig. 5

KAI2 and D14 protein characteristics. a We identified well-conserved positions in eu-KAI2 proteins (i.e. >70% of sequences have the same amino acid) in which the amino acid is characteristic of eu-KAI2 proteins (i.e. found in <30% of other sequences). These are listed at the left (position and amino acid). We then tested whether various clades share elements of this structure (i.e. how frequently the same amino acid is found at the same position in that clade). Charophyte and lycophyte KAI2 proteins are a close match, while KAI2B and KAI2E/F proteins from liverworts and mosses respectively have considerable similarity. However, DDK, D14 and DLK2 proteins do not share these characteristics. b We performed the same analysis with eu-D14 proteins, but only identified 7 characteristic residues. We thus extended the search to the combined D14-DLK4 clade and identified another 13 residues characteristic of the wider clade. These are listed at the left (position and amino acid). Very little conservation of these characteristic residues is found in other members of the DDK family