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Table 1 Acidic residues are rarer in TMHs of single-pass proteins than in TMHs of multi-pass proteins

From: Charged residues next to transmembrane regions revisited: “Positive-inside rule” is complemented by the “negative inside depletion/outside enrichment rule”

Dataset

Acidic residues (D and E)

Aspartic acid (D only)

Glutamic acid (E only)

μ SP

μ MP

H statistic P value

μ SP

μ MP

H statistic P value

μ SP

μ MP

H statistic P value

ExpAll

0.086

0.309

148.1 4.50E-34

0.045

0.157

40.3 2.13E-10

0.042

0.161

46.6 8.64E-12

UniHuman

0.076

0.398

316.5 8.31E-71

0.034

0.191

91.6 1.05E-21

0.042

0.207

100.3 1.33E-23

UniER

0.106

0.430

34.4 4.39E-9

0.061

0.161

8.0 4.72E-3

0.045

0.268

26.8 2.24E-7

UniGolgi

0.097

0.381

39.8 2.88E-10

0.043

0.180

19.4 1.05E-5

0.053

0.201

20.2 7.01E-6

UniPM

0.039

0.400

121.0 3.86E-28

0.016

0.187

32.7 1.06E-8

0.022

0.213

36.9 1.26E-9

UniCress

0.062

0.434

163.5 1.99E-37

0.036

0.198

32.5 1.20E-8

0.025

0.241

66.0 4.59E-16

UniFungi

0.177

0.349

43.1 5.14E-11

0.044

0.166

24.5 7.60E-7

0.133

0.183

4.6 0.033

UniBacilli

0.089

0.352

24.1 9.16E-7

0.048

0.185

11.2 8.27E-4

0.040

0.176

12.3 4.54E-5

UniEcoli

0.148

0.315

2.7 0.100

0.111

0.150

0.1 0.729

0.037

0.163

2.2 0.140

UniArch

0.438

0.606

1.8 0.183

0.083

0.344

11.2 8.33E-4

0.354

0.247

3.5 0.0624

  1. The statistical results when comparing the number of acidic residues in single-pass or multi-pass TMHs within their database-defined limits and excluding any flanks. The number of helices per dataset can be found in Table 2 for single-pass TMHs and Table 3 for multi-pass helices. µSP is the average number of the respective residues per helix in TMHs from single-pass proteins, while µMP is the average number of the respective residues per TMH from multi-pass proteins. The Kruskal-Wallis test scores (H statistics) were calculated for the numbers of aspartic acid and glutamic acid residues in each helix from single-pass and the number of aspartic acid and glutamic acid residues in each helix from multi-pass TMHs