Fig. 1From: Distinct unfolded protein responses mitigate or mediate effects of nonlethal deprivation of C. elegans sleep in different tissuesA periodic mechanical stimulus can partially and nonlethally reduce lethargus quiescence and induce translocation of DAF-16. a The fraction of quiescence measured during L4 lethargus of undisrupted (gray) and deprived (orange) wild-type animals and daf-16 mutants. Locomotion was forced using a square wave of mechanical vibrations with a 6-min period and 50% duty cycle. N = 30 animals, shaded areas depict mean ± standard error of the mean (SEM). b The total time in which quiescence was observed integrated over the data presented in a. Error bars depict mean ± SEM. c DAF-16::GFP fluorescence in the intestine before and after 1 h of sleep deprivation. Top: examples of green fluorescent protein (GFP) fluorescence in unperturbed and partially deprived animals. Arrowheads point to bright particles indicating concentration of DAF-16::GFP. Bottom: Histograms of the number of bright particles per animal identified under each set of conditions. Sample sizes are denoted in parentheses. Single and double asterisks denote significant differences with p < 0.05 and p < 0.01, respectivelyBack to article page