Fig. 4

QTL-seq-based analysis of sex determination in D. rotundata. a Male and female inflorescences of D. rotundata. Bars = 10 mm. b SNP-index and ∆SNP-index plots generated for pseudo-chromosome 11 (see Fig. 2). DNA samples from 50 male and 50 female F1 individuals were pooled to prepare the male and female bulks, respectively. Green, yellow, and blue dots represent SNP-index values at all SNP positions, and red lines denote the sliding window average SNP-index values at 1-Mb intervals with 50-kb increments. Horizontal brown lines in the ∆SNP-index plot represent the 95% confidence limit. The candidate genomic region presumably associated with sex determination is indicated by a pink background. c Schematic diagram showing the possible genotypes of female (P3, TDr97/00917) and male (P4, TDr97/00777) parents as well as their F1 progeny segregating for female and male. Genotypes of sex-determination locus are indicated as ZW or ZZ. The position of the cleaved amplified polymorphic sequence (CAPS) marker, sp1, is indicated by a dashed line. Sister chromatids are indicated by numbers