Fig. 2

PCNA-dependent enhancement of Mus81-Mms4 activity. a Reaction mixtures containing DNA substrate (5 nM), Mus81-Mms4 (0.25 nM), and increasing amounts of PCNA (0.25, 0.5, 1.25, and 2.5 nM, or 0.05, 0.1, 0.25, and 0.5 μM) were incubated at 37 °C for 20 min and then analyzed. b Quantification of data from three independent experiments. c Effect of PCNA on Mus81-Mms4 nuclease activity on various DNA substrates. Mus81-Mms4 (0.2 nM) was incubated in the absence or presence of PCNA (0.5 μM) at 37 °C for the indicated time. The data from three independent experiments were quantified. *Raw data provided in Additional file 2