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Fig. 8 | BMC Biology

Fig. 8

From: An intrinsically disordered linker controlling the formation and the stability of the bacterial flagellar hook

Fig. 8

Analysis of the effect of mutations in the structural context of the hook. Analysis of the different mutations in the ID-Rod-Stretch: (a) Lys32 of S. enterica FlgE, a conserved residue of the ID-Rod-Stretch, is located in a pocket with Asp62, Arg95, and Glu361 in this theoretical model. Both Arg95 and Glu361 are conserved in the FlgE protein family. b Two of the three molecules of FlgE, at position “-5” (cyan) and “-11” (light brown), which interact with the modeled ID-Rod-Stretch of the flagellar hook of S. enterica. Mutations in the ID-Rod-Stretch that are in the interactive domains will affect the stability of the hook. The cryo-electron microscopy structure, including ID-Rod-Stretch of FlgE from C. jejuni [19] (c), is longer than in S. enterica and it is shown here interacting with four FlgE molecules in positions “-5” (cyan), “-10” (green), “-11” (light brown), and “-16” (purple). d Reducing the length of the disordered segment of C. jejuni FlgE reduces its number of interactions with other FlgE molecules in the hook. This has a negative effect on the cohesion of the hook

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