Fig. 2
a Expression of claudins in different cell types normalized to that in TYP organoids (=1.0). i Cldn1 seemed greatest in GOBs and PANs (n = 4 ISC; n = 3 others). ii Cldn2 was highest in ISCs (n = 4 ISCs; n = 3 others). iii Cldn7 was high in ENTs (n = 4 ENTs; n = 3 others). iv Cldn15 was relatively homogenous (n = 4 ENTs; n = 3 others). Pa,b ,c,d ≤ 0.05. b,c Expression of TJ proteins in different cell types. b,i Occludin (Ocln) was higher in ISCs and PANs (n = 5 ISCs; n = 3 others). ii ZO-1 (Tjp1) was similar among organoids (n = 4 for ISCs, ENTs; n = 3 others). iii Epithelial cadherin 1 (Cdh1) was relatively high in PANs (n = 4 ENTs; n = 3 others). iv Myosin light chain kinase (Mylk) was lowest in ISCs (n = 3). c,i Tricellulin (Marveld2) was lowest in ENTs (n = 4 TYPs, ISCs, ENTs; n = 3 others). ii Cingulin (Cgn) expression was high in PANs and CRYs (n = 5 ENTs; n = 3 PANs, CRYs; n = 4 others). iii Expression of junctional adhesion molecule-1 (Jam1) and iv JAM4 (Jam4) were lowest in ISCs (n = 5 ENTs; n = 4 TYPs, ISCs, GOBs; n = 3 others). d Immunofluorescence of representative TJ proteins in organoids probed with ZO-1 (green), ECAD (red), CLDN2 (CL2; red), CLDN7 (CL7; green), or OCLN (green) (60×, bars = 25 μm). e Cellular location. ZO-1 staining is mainly in the apical area and ECAD in the basolateral area. CLDN2 is found along the brush border of ISCs and PANs but not in ENTs and GOBs. CLDN7 seems basolateral but expressed less in ISCs. OCLN seems apical. Supporting data sets are deposited in the figshare repository [50]. Cdh or ECAD, E-cadherin, Cgn cingulin, Cldn or CL claudin, CRY crypt, ENT enterocyte, GOB goblet cell, HOM homogenates of intestinal mucosa, ISC intestinal stem cell, Jam, junctional adhesion molecule, L lumen, Marveld2 MARVEL domain containing 2, Mylk myosin light chain kinase, Ocln occludin, PAN Paneth cell, Tjp or ZO1 tight junction protein, TYP typical