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Fig. 4 | BMC Biology

Fig. 4

From: Marked differences in tight junction composition and macromolecular permeability among different intestinal cell types

Fig. 4

Dextran accumulation in ENT, PAN, and ISC organoids over time. ENT, PAN, and ISC organoids were each exposed to 4 kDa dextran for 10, 30, and 60 min, washed, and then imaged with an inverted confocal DMi8 microscope equipped with a CSU-W1 spinning disk (see text). Then, 5-mm Z-stacks were acquired above and below the vertical midpoint of the organoid. Representative images from above (i, ii, iii) and below (iv, v, vi) the midpoint of ENT (a), PAN (b), and ISC (c) organoids at 10, 30, and 60 min intervals (n = 5 or 6 organoids). Dextran accumulation was clearly greater in ENT organoids at all time points. Luminal dextran levels increased with incubation time. Supporting data sets are deposited in the figshare repository [50]. ENT enterocyte, ISC intestinal stem cell, PAN Paneth cell

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