Skip to main content
Fig. 7 | BMC Biology

Fig. 7

From: Identification of a neuronal population in the telencephalon essential for fear conditioning in zebrafish

Fig. 7

Behavioral analyses of the SAGFF120A;UAS:zBoTxBLC:GFP fish. ac The light response of wild type (n = 7) (a) and the SAGFF120A;UAS:zBoTxBLC:GFP (n = 7) (b) fish. Means ± SEM are plotted. c The maximum locomotor activities 100 ms before (Before) and after (After) light-on are plotted with Tukey box plot. Wilcoxon signed rank test was performed for the same group (P = 0.0313 for wild type and P = 0.0156 for SAGFF120A;UAS:zBoTxBLC:GFP fish). Mann–Whitney U test was performed between two groups (P = 0.2086 for “Before”, P = 0.2593 for “After”). ns, not significant. d Comparison of the locomotor activity of wild type (n = 9) and SAGFF120A;UAS:zBoTxBLC:GFP (n = 7) fish with Tukey box plot. The Mann–Whitney U test was performed (P = 0.8372). e Pavlovian fear conditioning. Wild type fish (n = 7) showed increased turning activities in response to CS after conditioning. SAGFF120A;UAS:zBoTxBLC:GFP (n = 7) fish showed significantly reduced turning activities. Mean ± SEM and individual values are plotted. Two-way ANOVA, genotype (wild type, double transgenic) × conditioning (before, after) (F = 16.10, P = 0.0005), and Tukey’s multiple comparison post-hoc tests were performed (***P < 0.001). fi The alarm response of wild type (n = 9) (f) and SAGFF120A;UAS:zBoTxBLC:GFP fish (n = 8) (g). (f, g) The speed of fish before and after addition of the skin extract. Mean ± SEM are plotted. The locomotor activities were divided into three phases; B (before addition of the skin extract), EM (erratic movement), and PEM (post-erratic movement). h Comparison of the speeds during B, EM, and PEM with Tukey box plot. Mean is marked by ‘+’. Unpaired t-test with Welch’s correction was performed (*P < 0.05). i Comparison of freezing during PEM. Mean is marked by ‘+’. Unpaired t-test with Welch’s correction was performed (*P < 0.05.)

Back to article page