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Fig. 3 | BMC Biology

Fig. 3

From: Using in vivo oxidation status of one- and two-component redox relays to determine H2O2 levels linked to signaling and toxicity

Fig. 3

Kinetics of Tpx1 and HA-OxyR oxidation in wild-type and Δtrx1 cells. Cultures of strains AD29 (WT) (a, b) or AD61 (Δtrx1) (c, d), expressing HA-OxyR, were treated or not with the indicated concentrations of H2O2 for 1 min. Protein extracts were obtained and processed as described in Fig. 1b using antibodies against Tpx1 (a, c) or HA (b, d). Reduced and oxidized Tpx1 and OxyR are indicated with arrows. The percentages of oxidized Tpx1 or HA-OxyR are indicated with bars (right panels). Error bars (standard error of the mean) from three independent biological replicates are shown. WT wild type

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