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Fig. 2 | BMC Biology

Fig. 2

From: Ultrastructural analysis of mitotic Drosophila S2 cells identifies distinctive microtubule and intracellular membrane behaviors

Fig. 2

Schematic representation of intracellular membrane behavior during mitosis of S2 cells. a Membrane remodeling during prometaphase and metaphase. In PM1, a QNM begins to form in the proximity of the nuclear fenestration area, and during PM2, it extends along the entire nuclear envelope. During late PM2 and PM3, the inner double membrane of the QNM becomes swollen and progressively disassembles through a vesiculation process, while a RDM persists around the nucleus. In PM4, only small patches of QNM are observed at the cell poles and the RDM disassembles. In metaphase, multilayer stacks of ER membranes form alongside the spindle. b Membrane length measured in 70 nm sections of interphase and mitotic cells: interphase/prophase (seven cells), PM1 (nine cells), PM2 (seven cells), PM3 (15 cells), PM4 (13 cells), metaphase (12 cells), and anaphase (seven cells). The DNM is present in interphase and prophase cells, in PM1 cells, and around some regions of anaphase chromosomes. In PM3 cells, the DNM is no longer present and the non-QNM portions of the nuclear envelope are made of RDM. To calculate the total membrane length, we counted the QNM twice. Note that the total membrane length stays mainly constant from prophase through to late prometaphase, and increases in metaphase and anaphase. Error bars are for the standard error of the mean. DNM double nuclear membrane, ER endoplasmic reticulum, QNM quadruple nuclear membrane, RDM residual double membrane

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