|  |  |  |  |  | F0 with: |
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MS | Donor type | Guide ID(s) | Donor ID | Embryos transferred | F0 biopsied (birth rate) | Mutation | Correct mutation | SM only | SM only and rearranged | NHEJ alleles | Random integration |
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1 | ssODN | 20 | Gckrdonor_20 | 80 | 12 (15%) | 0 | 0 | 0 | 0 | 0 | n.d. |
2 | ssODN | 3 | Gckrdonor_2 | 80 | 21 (26%) | 12 | 0 | 2 | 2 | 9 | n.d. |
3 | ssODN | 3 | Gckrdonor_2 | 70 | 13 (19%) | 4 | 0 | 0 | 0 | 4 | n.d. |
4 | ssODN | 3 | Gckrdonor_2 | 135 | 18 (13%) | 9 | 0 | 2 | 1 | 7 | n.d. |
5 | ssODN | 3 | Gckrdonor_2 | 42 | 10 (23%) | 3 | 0 | 0 | 0 | 3 | n.d. |
6 | ssODN | 3 | Gckrdonor_3 | 121 | 8 (7%) | 5 | 0 | 1 | 1 | 3 | n.d. |
7 | ssODN | 3 | Gckrdonor_3 | 112 | 8 (7%) | 3 | 0 | 0 | 0 | 3 | n.d. |
1 | lssDNA | 5.2, 3.1 | Gckr_P446L_lss | 210 | 22 (10%) | 14 | 8 | 1 | 2 | 7 | 0/2 |
- The table shows the numbers of embryos and animals involved in mutagenesis attempts employing the injection of CRISPR/Cas9 reagents and oligonucleotides or lssDNA donors. The percentage of transferred embryos yielding live animals at weaning is shown in parentheses. The outcome of these attempts is also summarized. Note that sgRNA_20 was employed for the first microinjection session with ssODN_20 and substituted to sgRNA_3 and relevant donor ssODNs for subsequent sessions, as it was confirmed to be inactive. Sequencing data from this project are displayed in Fig. 4 (additional raw sequencing data are provided in Additional file 16)
- MS microinjection session, n.d. not determined SM silent mutation