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Fig. 2 | BMC Biology

Fig. 2

From: Dehydration triggers ecdysone-mediated recognition-protein priming and elevated anti-bacterial immune responses in Drosophila Malpighian tubule renal cells

Fig. 2

Malpighian tubule (MT) EcR and PGRP-LC induced by desiccation and 20E feeding. Relative mRNA of (a) EcR and (b) PGRP-LC in control genotype (+/EcR(RNAi)) and with EcR(RNAi) expressed in stellate cells (c724>) or principal cells (c324>). c Relative mRNA of PGRP-LC in control genotype (+/eip75B(RNAi)) and when eip75B(RNAi) is expressed in stellate cells (c724>) or principal cells (c324>). a–c Mean with SEM of three independent biological samples for each genotype, normalized relative to food treatment, wild-type genotype. Within each genotype: one-way ANOVA with treatment as main effect (N = 9), *p < 0.05, **p < 0.01 and Tukey’s post hoc comparison. d Relative mRNA for PGRP-LC, EcR, Defensin, and Cec-A2 from MTs of adults fed 20E, each normalized to corresponding control. Mean of three independent biological samples (SEM); t test for each gene with Holm–Bonferroni Sequential correction *p < 0.05

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