Fig. 4

Peptidoglycan recognition-protein priming increases with age. (a) EcR and (b) PGRP-LC mRNA from Malpighian tubules (MTs) of young (7 days) and old (40 days) yw females in control (food, PBS) and desiccation treatments. Expression was normalized relative to young flies in the food treatment control; mean of three independent biological samples with SEM; two-way ANOVA with age and treatment as main effects, Tukey’s post hoc comparison, *p < 0.05, **p < 0.01. c 20E extracted from 7- and 40-day-old wild-type females (yw and wDah) in control (food, PBS) and desiccation treatments; mean of three independent biological samples with SEM. Within each genotype, two-way ANOVA with age and treatment as main effects, Tukey’s post hoc comparison, *p < 0.05, **p < 0.01, ***p < 0.001. d Disembodied (dib) mRNA from specific tissues of old females (40 days) in control (food, PBS) and desiccation treatments; normalized relative to food treatment, head tissue of young females; mean of three independent biological samples for each tissue type. Statistical comparisons within each tissue by t test for difference between desiccation and PBS treatment: dib mRNA was significantly elevated in MTs (*p < 0.05 with Holm-Bonferroni Sequential correction)