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Table 1 Examples of synchrotron and XFEL sources for serial crystallography methods

From: Entering an era of dynamic structural biology

 

Diamond light source

XFELs (~ 50 fs pulse duration)

Beamline

I04

I24

VMXi

Eu.XFEL

LCLS-II

SACLA

SwissFEL

PAL XFEL

Photons/time

5 × 1011

(s−1)

3 × 1012

(s− 1)

> 1014 a

(s− 1)

~ 1012

(pulse− 1)

≥ 1012

(pulse− 1)

2 × 1011

(pulse− 1)

~ 7 × 1011

(pulse− 1)

2 × 1011

(pulse− 1)

Beam size (μm2)

~ 10 × 5

~ 5 × 5

≤ 5 × 5

< 1–10 × 10

< 1–3 × 3

1.3 × 1.3

< 3 × 3

< 3 × 3

Detector/pulse (Hz)

25–100 (750) b

25–100

750 b

27,000 c

120– 10 6 d

60

100

60

  1. aUsing a double multilayer monochromator (double crystal monochromator DCM > 2 × 1012 ph/s)
  2. bDectris Eiger 4 M or region of interest within Eiger 16 M
  3. c2700 pulses in a 600-μs train (4.5 MHz) at 10 Hz
  4. dLCLS-II (~ 2020, soft X-rays at 106, hard at 120 Hz) and LCLS-II-HE (~ 2022, soft and hard X-rays at 106 Hz)