Unique sperm haplotypes are associated with phenotypically different sperm subpopulations in Astyanax fish

Table 2 The distribution of haplotypes among the subpopulations of sperm

2a All three subpopulations (χ² = 105.3, df = 6, p < 1E−6)
Observed	M_C O_S	M_S O_C	M_C O_C	M_S M_O
Pop1	44	10	19	48
Pop2	5	99	18	33
Pop3	21	47	19	24
Expected	M_C O_S	M_S O_C	M_C O_C	M_S M_O
Pop1	21.9	48.8	17.5	32.8
Pop2	28.0	62.5	22.4	42.1
Pop3	20.1	44.7	16.1	30.1
Standardized residuals (z)
	M_C O_S	M_S O_C	M_C O_C	M_S M_O
Pop1	4.7	−5.6	0.4	2.6
Pop2	−4.4	4.6	−0.9	−1.4
Pop3	0.2	0.3	0.7	−1.1
2b Sub populations 2 and 3 merged (χ² = 87.7, df = 3, p < 1E−6)
Observed	M_C O_S	M_S O_C	M_C O_C	M_S M_O
Pop1	44	10	19	48
Pops2&3	26	146	37	57
Expected	M_C O_S	M_S O_C	M_C O_C	M_S M_O
Pop1	21.9	48.8	17.5	32.8
Pops2&3	48.1	107.2	38.5	72.2
Standardized residuals (z)
	M_C O_S	M_S O_C	M_C O_C	M_S M_O
Pop1	4.7	−5.6	0.4	2.6
Pops2&3	−3.2	3.7	−0.2	−1.8

The distribution of the four haplotypes among the subpopulations of sperm is not random. The haplotypes are Mc1r Cave/Oca2 Surface (M_C O_S), Mc1r Surface/Oca2 Cave (M_S O_C), Mc1r Cave/Oca2 Cave (M_C O_C), and Mc1r Surface/Oca2 Surface M_S M_O. Entries in italics are considered significant with z > 2

ISSN: 1741-7007