Fig. 5

Knock-down of SSU-1 (ssu-1) reduces lifespan in wild-type and daf-16(mu86)-mutant C. elegans. a The orthologous mammalian phase-II conjugation enzyme SULT1C3 is significantly more abundant in NMR compared to GP, and it declines with aging. b Wild-type (N2, Bristol strain) and c daf-16(mu86)-mutant worms were treated with ssu-1 and control empty vector (e.v.) RNAi from the L4 (pre-adult) developmental stage. Survival was scored daily; the significance of lifespan shortening was determined by Mantel-Cox Log rank test (p < 0.0001 in both cases). The N2 experiment was repeated five times and the daf-16(mu86) two times with same results. For each repetition, ≥ 140 animals per condition were used. One representative replicate is shown for each experiment. Related to Additional file 11: Figure S4