Fig. 8

Effect of delipidation on MelB_St binding and stability. MelB proteins purified from varied lipids strains were subjected to the delipidation treatment as described in Methods. a Galactoside binding by the Trp→D²G FRET assay. The delipidated MelB_St at 1 μM was used to test binding of galactosides by Trp→D²G FRET assay based on a time trace. b Determination of T_m value by CD spectra. The delipidated MelB_St at 10 μM in a buffer containing 20 mM NaPi, 100 mM NaCl, 10% glycerol and 0.01% DDM are used for thermal denaturation test by monitoring CD ellipticity changes at 210 nm between temperatures 25–90 °C