Skip to main content

Table 1 Strains and plasmids

From: Structural and functional characterization of protein–lipid interactions of the Salmonella typhimurium melibiose transporter MelB

 

Genotype or description

Reference

E. coli strain

 AL95 (PE deficiency)

pss93::kanR lacY::Tn9

[33]

 MG1655 (WT)

F lambda ilvG rfb 50 rph-1

[34]

 UE54

(PG deficiency & CL deficiency)

MG1655 lpp-2 Δara714 rcsF::miniTn10cam ΔpgsA::FRT-Kan-FRT

(pgsA encodes phosphatidylglycerol phosphate synthase)

[34]

 WK3110 (WT)

F lambda IN(rrnDrrnE)

[33]

 BKT12

(CL deficiency)

WK3110 ΔclsA, ΔclsB, ΔclsC::KanR

(cardiolipin synthases (Cls) catalyze the condensation of two PG molecules to one CL and one glycerol)

[35]

 DW2

melA+ ΔmelB ΔlacZY

[37]

Plasmid

 pK95 ΔAH/MelBSt/CHis10

MelBSt with a C-terminal His10 tag (constitutive expression; ampicillin resistant).

[6, 37]

 pDD72GM

pssA+ genR and pSC101 temperature-sensitive replicon (IPTG induction; chloramphenicol resistant).

[20]

  1. [Note that clsC uses PG and PE to make CL plus ethanolamine]