Genotype or description | Reference | |
---|---|---|
E. coli strain | ||
AL95 (PE deficiency) | pss93::kanR lacY::Tn9 | [33] |
MG1655 (WT) | F− lambda− ilvG− rfb− 50 rph-1 | [34] |
UE54 (PG deficiency & CL deficiency) | MG1655 lpp-2 Δara714 rcsF::miniTn10cam ΔpgsA::FRT-Kan-FRT (pgsA encodes phosphatidylglycerol phosphate synthase) | [34] |
WK3110 (WT) | F− lambda− IN(rrnD−rrnE) | [33] |
BKT12 (CL deficiency) | WK3110 ΔclsA, ΔclsB, ΔclsC::KanR (cardiolipin synthases (Cls) catalyze the condensation of two PG molecules to one CL and one glycerol) | [35] |
DW2 | melA+ ΔmelB ΔlacZY | [37] |
Plasmid | ||
pK95 ΔAH/MelBSt/CHis10 | MelBSt with a C-terminal His10 tag (constitutive expression; ampicillin resistant). | |
pDD72GM | pssA+ genR and pSC101 temperature-sensitive replicon (IPTG induction; chloramphenicol resistant). | [20] |