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Fig. 3 | BMC Biology

Fig. 3

From: UBN1/2 of HIRA complex is responsible for recognition and deposition of H3.3 at cis-regulatory elements of genes in mouse ES cells

Fig. 3

Residues Ala87 and Gly90 of H3.3 are important for recognition and binding of H3.3 by HIRA complex. a, b Both Ala87 and Gly90 of H3.3 are required for binding UBN1. Top panel, schematic diagram shows the different amino acid residues between H3.1 and H3.3; Bottom panel, interaction between UBN1 subunit and H3.1 or H3.3 mutants is analyzed by LacO-LacI targeting system (a) or Western blot of anti-Flag immunoprecipitates (b). Statistic results are shown in Additional file 1: Figure S3C. Scale bar, 10 μm. (c, d) Ala87 and Gly90 of H3.3 are sufficient to confer the specificity toward UBN1. Interaction between UBN1 subunit and H3.1 mutants is analyzed by LacO-LacI targeting system (c) and Western blot of anti-Flag immunoprecipitates (d). Statistic results are shown in Additional file 1: Figure S3D, Scale bar, 10 μm

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