Fig. 1
From: VOPP1 promotes breast tumorigenesis by interacting with the tumor suppressor WWOX
WWOX and VOPP1 expression, interaction, and binding domains. a Co-immunoprecipitation of Myc-WWOX and Flag-VOPP1 in HEK-293T cells. Antibodies for immunoprecipitation (IP) and immunoblotting (IB) analyses are indicated. VOPP1 mRNA (b) and protein (c) expression in ten breast cancer cell lines. d Co-immunoprecipitation of the endogenous WWOX/VOPP1 complex. MDA-MB-468 cells were immunoprecipitated with either normal rabbit IgG as a negative control (IgG) or anti-VOPP1 antibody (VOPP1). Immunoprecipitates were examined by immunoblotting with anti-WWOX antibody. e Co-immunoprecipitation with wild-type and mutants forms of WWOX and VOPP1 constructs WWOXY33R, VOPP1Y119A, VOPP1Y157A, and VOPP1Y165 were performed as indicated in HEK-293T cells. WCL, whole cell lysate