Fig. 2

mRNA-seq reveals transcriptional effects of food perception. a PCA of four biological replicates is plotted. Ellipses represent 80% confidence intervals, the probability for two of which not overlapping by chance is approximately 0.04. b, c Mean gene expression changes of four biological replicates are plotted for the indicated conditions. d Overlap between genes significantly affected by food at 1 h, ivermectin and food at 1 h, and ivermectin and food at 24 h is plotted. e Mean gene expression changes of two to four biological replicates is plotted for the indicated conditions and genotypes. The universal set of genes considered includes only those analyzed in both studies. f Overlap between genes significantly affected by ivermectin and food with genes significantly affected by daf-16 is plotted with the same universal set as in e. a–f Quadruple mutant transgenic background, except for daf-16 in wild-type background