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Fig. 6. | BMC Biology

Fig. 6.

From: Detecting neural assemblies in calcium imaging data

Fig. 6.

Application of the different algorithms to stimulus-evoked calcium imaging data from the larval zebrafish optic tectum. a 11 different stimuli were shown to the fish. The stimuli were separated by 15° in the visual field of the fish. b The deflection of calcium fluorescence from baseline level, \(\frac {\Delta F}{F}\), for the 160 neurons over about 180 s of the recording. The neurons are ordered by their anterior-posterior position in the tectum. The stimuli were presented in the order 11 – 1 – 10 – 2 – 6 – 3 – 8 – 4 – 9 – 5 – 7 as indicated. c Example calcium trace over the course of the whole experiment from a neuron particularly responsive to stimulus 11, whose onset is indicated. The overall noise is relatively low and the peaks in fluorescence are clearly visible. d The average population response in terms of fluorescence (\(\frac {\Delta F}{F}\)) to the 11 different stimuli. The responses to the first 3 stimuli were weak compared to the others. e–j Graphical representations of the assemblies recovered by the different algorithms. The neurons which were part of the respective assemblies are marked in black. e SGC recovered 8 assemblies. f ICA-CS recovered 5 assemblies. g Promax-CS recovered 5 assemblies. h SVD recovered 5 assemblies. i CORE recovered 1 assembly. j ICA-MP recovered 2 assemblies. k Promax-MP recovered 16 assemblies. l FIM-X recovered 27 assemblies

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