Fig. 1

Cas12a and Cas9 nuclease activities in rice protoplasts under different temperatures: 22 °C, 28 °C, 32 °C, and 37 °C. Percentage of mutations by AsCas12a, FnCas12a, and LbCas12a at OsROC5 (a) and OsDEP1 (b), and by SpCas9 at OsPDS (c). GFP-transfected samples were used as controls. Error bars represent standard deviations of two biological replicates