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Fig. 5 | BMC Biology

Fig. 5

From: GSK-3β protects fetal oocytes from premature death via modulating TAp63 expression in mice

Fig. 5

GSK-3β regulated nuclear translocation and transcriptional activation of β-catenin in the fetal ovary. a Inhibition of GSK-3β impaired phosphorylation of β-catenin in the fetal ovary. Western blotting showed a significant decrease in the phospho-β-catenin protein level following GSK-3β inhibition. GAPDH was used as an internal control. b β-catenin accumulated in the cytoplasm and translocated to the nucleus following GSK-3β inhibition. Immunofluorescence staining for β-catenin (green) and DDX4 (red). The nucleus was stained by Hoechst (blue). β-catenin displayed cytoplasmic accumulation and expression in the oocyte nucleus (arrowhead) following GSK-3β inhibition. c qRT-PCR analysis showed that mRNA levels of several canonical target genes of β-catenin (normalized to β-actin) increased significantly following GSK-3β inhibition (Additional file 8: Individual data values). The data are presented as mean ± s.d. The asterisk (*) denotes a statistically significant difference between the control and treatment groups. *P < 0.05, **P < 0.01, and ***P < 0.001 (t test). Scale bars, 200 μm

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