Fig. 7
From: GSK-3β protects fetal oocytes from premature death via modulating TAp63 expression in mice
β-catenin induced TAp63 transcriptional activation in vivo. a The expression of β-catenin in fetal and neonatal mouse ovary in vivo. Mouse ovaries from 13.5 dpc, 15.5 dpc, 17.5 dpc, and 1 dpp were immunostained for β-catenin (green) and DDX4 (red). The nucleus was stained by Hoechst (blue). β-catenin became intensively accumulated in the oocyte cytoplasm and nucleus from 17.5 dpc onward. b The expression of active β-catenin in fetal and neonatal mouse ovary in vivo. Active β-catenin began to express in the majority of the oocyte nucleus from 17.5 dpc onward. c ChIP-qPCR analysis of the interaction between β-catenin and TAp63 promoters sequences in 17.5 dpc mouse ovaries (Additional file 8: Individual data values). d Schematic model depicting the essential role of GSK-3β in maintaining fetal oocyte survival during meiotic prophase I. The data are presented as mean ± s.d. The asterisk (*) denotes a statistically significant difference between the control and treatment groups. *P < 0.05, **P < 0.01, and ***P < 0.001 (t test). Scale bars, 200 μm