Fig. 3

MGX vs. SurfCut signal layer extraction: principles, pros, and cons. Illustration of the principles of the signal layer extraction for MGX and SurfCut on the highly curved shoot apical meristem. a Schematic representation of the orthogonal view of the shoot apical meristem. Only the epidermal cell layer is drawn. b The surface signal is detected (blue) from the raw 3D confocal signal (black) and used as a basis to crop the raw signal at a given depth (orange), either c perpendicular to the surface (MGX) or d in the z-axis (SurfCut)