Fig. 1
From: Biological and RNA regulatory function of MOV10 in mammalian germ cells
Expression and localization patterns of MOV10 in male germ cells. a Time-course western blot analysis of MOV10 in postnatal mouse testes, compared with MOV10L1 and LIN28. TUBULIN was used as a loading control. Mouse testes were collected from P2 through adulthood. b Expression patterns of MOV10, MOV10L1, and LIN28 in different populations of male germ cells. Spg, spermatogonia; PS, pachytene spermatocytes; RS, round spermatids. c Western blot analysis of MOV10 in cytoplasmic and nuclear fractions of P10 testis. GAPDH and H3 were used as cytoplasmic and nuclear markers, respectively. MILI and MVH are known to be present in perinuclear granules in the cytoplasm. d Whole-mount immunostaining for MOV10 and PLZF in seminiferous tubules. Image shows As, Apr, and Aal (A4, A8, A16) clusters of spermatogonia that are interconnected by intercellular cytoplasmic bridges due to incomplete cytokinesis. Dotted lines mark clusters of PLZF (shown in red) and MOV10 (shown in green) double-positive cells, counterstained with DAPI (shown in blue). Scale bars, 50 μm. e–g Frozen sections of testes from P10 (e), Spg isolated from P6–8 testes (f), and in vitro cultured SPCs (g) were co-immunostained for MOV10 and PLZF. Panel e shows high-magnification images of regions marked by dashed lines. Scale bar, 20 μm