Skip to main content
Fig. 2 | BMC Biology

Fig. 2

From: Biological and RNA regulatory function of MOV10 in mammalian germ cells

Fig. 2

Mov10 knockdown affects cell fate decisions in SPC. a Morphological differences between Mov10 shRNA (shMov10-832 or shMov10-833) and control (shVector) virus transduced SPC cultures at 6 days following lentiviral transduction. Mov10 shRNA cultures exhibit dissociation of clump-forming cells and contain only few and small grape-shaped colonies. Scale bar, 20 μm. b Significantly lower cell numbers in SPC cultures transduced with Mov10 shRNA vs cells transduced with control virus. c, d Flow cytometric analysis of apoptosis (c) and cell cycle (d). The error bars in b, c, and d represent variation (SEM) among biological triplicates. e, f Reduced repopulation capacity of SPCs after Mov10 knockdown. Mov10 shRNA or control vector transduced cells were transplanted into total 13 recipient testes. Donor-derived spermatogenic colonies (beta-glucuronidase transgene positive) were visualized by X-gal staining and counted. Scale bar, 2 mm. *p < 0.05; **p < 0.01; ***p < 0.001

Back to article page