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Fig. 7 | BMC Biology

Fig. 7

From: Biological and RNA regulatory function of MOV10 in mammalian germ cells

Fig. 7

Identification of MOV10-interacting proteins in the nucleus. Western blot (a) and silver staining (b) of the MOV10 IP complex from nuclear lysate. c Gene ontology analysis of MOV10-associated proteins in the nucleus. d Nine MOV10-associated splicing-related proteins were selected for validation by IP-western blot assay. The piRNA pathway proteins MILI and MOV10L1 served as negative controls. e Venn diagram showing the cross analysis of four sets of MOV10 nuclear IP-MS data. The purple line marks 32 proteins that were reproducibly identified in IP using RIPA buffer without RNase treatment and at least one other IP with RNase treatment. f Gene ontology analysis of the 32 reproducible MOV10-assoicated proteins. g FLAG-MOV10 and HA-target (SRSF1, DDX5 or DDX17) were overexpressed in HEK293T cells, and IPs were performed by FLAG and HA antibodies, followed by western blot analysis

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