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Fig. 12. | BMC Biology

Fig. 12.

From: Regeneration in the ctenophore Mnemiopsis leidyi occurs in the absence of a blastema, requires cell division, and is temporally separable from wound healing

Fig. 12.

Regenerative ability is recovered after HU treatment ends. A Reversible S-phase arrest after HU treatment as detected by EdU labeling performed at the indicated time points (red arrows). Nuclei of S-phase cells are labeled with EdU (magenta), and all nuclei are counterstained with DAPI (blue). Note that cells have already resumed cell cycle progression 1 day after HU wash. B, E Schematic representation of HU treatment and wash experiment in bisected cydippids through the oral-aboral axis (B) and cydippids in which the apical organ was amputated (E). C, C’ DIC images of bisected cydippids in lateral view showing the wound site at 48 hab. Note that there is no sign of regeneration of the missing structures. D, D’ DIC images of bisected cydippids in lateral view showing the wound site at 96 hab. Note that all missing structures (comb rows and tentacle bulb) are regenerated. The dotted line rectangle in C and D shows the area corresponding to higher magnification at the bottom. FG’ DIC images of amputated cydippids oriented in a lateral (top panels) and aboral (bottom panels) view showing the wound site at 48 hpa. Note that there is no sign of regeneration of the missing structures. HI’ DIC images of amputated cydippids oriented in a lateral (top panels) and aboral (bottom panels) view showing the wound site at 96 hpa. Note that all components of the apical organ are regenerated. Dotted line rectangles in F, G, H, and I show the area corresponding to higher magnification on the right. Scale bars = 100 μm. Abbreviations: hab hours after bisection, hpa hours post amputation, ao apical organ, cr comb row, tb tentacle bulb, dc dome cilia, l lithocyte, sc supporting cilia, ao fl apical organ floor, ec endodermal canal, cf ciliated furrows

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